Available technologies

Portfolio of human colorectal adenocarcinomas

Reference number: 5129

A portfolio of human colorectal adenocarcenoma cell lines including primary cell lines isolated from patients (of various Duke's stages and histological grades) in addition to cloned cells lines genetically modified to highly express alpha integrin domains.

Technology overview

Primary

Six cell lines from primary human colorectal adenocarcinomas were established from surgical specimens without the use of 'feeder' cells, 'conditioned' medium or passage of cells in nude mice. The six cell lines exhibit considerable variation in morphology, CEA secretion and tumourigenicity in nude mice. At least two of the lines retain some of the differentiated characteristics of colorectal epithelium. HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.

Clonal

HRA-19a1.1 is a clone derived from HRA-19. The HRA-19a1.1 cell line was transfected with alpha integrin domain coding plasmids to create cell lines highly expressing alpha2 or alpha2 and alpha 1 integrin. In experiments, cell lines expressing alpha2 showed a significantly increased endrocrine and mucous cell lineage commitment relative to the parent cell line.

Benefits

  • Cell lines are invaluable tools for biomedical research.
  • Cancer cell lines are the foundation of cancer biology and the quest for drug treatments.
  • Adenocarcinoma is a cancer of the epithelium (including colon) and originates in glandular tissue, such as skin surface layer and glands.

These cell lines are available through Public Health England ECACC. Please make your purchase through the links below.

Purchasing links through PHE

Publications

S C Kirkland and H Ying (2008) α2β1 Integrin regulates lineage commitment in multipotent human colorectal cancer cells, J Biol Chem, 283(41): 27612-27619.

Inventor information

Dr Sue Kirkland

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contact

Alexandra Skeaping

Industry Partnerships and Commercialisation Officer, Medicine

a.skeaping@imperial.ac.uk

+44 (0)20 7594 7021

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